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US 9,005,966 B2Granted
Generation of pluripotent cells from fibroblasts
AssigneeThe Regents of the University of California
InventorsWilliam E. Lowry, Kathrin Plath
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Abstract
Provided are methods and compositions useful for producing and propagating stem cells from fibroblasts.
Claims9
A method comprising:
- contacting a human somatic cell with at least one retroviral vector comprising polynucleotides encoding at least four de-differentiation factors selected from the group consisting of a (i) KLF4, (ii) OCT4, (iii) SOX2, (iv) c-MYC or n-MYC, and (v) NANOG,
- culturing the somatic cell to express the at least four de-differentiation factors;
- selecting cells that express a Tumor Rejection Antigen 1-61 and/or 1-81,
- subculturing the selected cells to obtain an enriched population of de-differentiated/induced stem cells.
1 more element
A method of generating a human stem cell comprising contacting a somatic cell with a retroviral vector comprising a set of polynucleotides encoding at least four de-differentiation factors selected from the group consisting of a KLF4, OCT4, SOX2, c-MYC or n-MYC, NANOG or any combination thereof, under conditions wherein the cell is infected with the retroviral vector;
- culturing the infected cell under conditions for expression of the polynucleotides encoding the at least four de-differentiation factors;
- and isolating cells expressing a TRA-1-81 and/or TRA-1-61 and a reduced or loss of expression of a vector gene to obtain human stem cells.
Dependencies are inferred from the claim text.