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US 9,005,924 B2Granted
Acceptor framework for CDR grafting
AssigneeESBATech AG
InventorDominik Escher
Life of this patent
Estimating the term…
Abstract
The present invention relates to an antibody acceptor framework and to methods for grafting non-human antibodies, e.g., rabbit antibodies, using a particularly well suited antibody acceptor framework. Antibodies generated by the methods of the invention are useful in a variety of diagnostic and therapeutic applications.
Claims4
A method of humanizing a rabbit immunobinder, the method comprising:
- (a) grafting the CDR H1, CDR H2 and CDR H3 sequences from a donor rabbit immunobinder into the human heavy chain acceptor framework comprising SEQ ID NO:
- 1;
- and
- (b) grafting the CDR L1, CDR L2 and CDR L3 sequences from a donor rabbit immunobinder into a human light chain acceptor framework into a light chain acceptor framework that has at least 85% identity to SEQ ID NO:
- 2.
2 more elements
A method of humanizing a rabbit immunobinder, the method comprising:
- (a) grafting the CDR H1, CDR H2 and CDR H3 sequences from a donor rabbit immunobinder into the human heavy chain acceptor framework comprising SEQ ID NO:
- 1;
- and
- (b) grafting the CDR L1, CDR L2 and CDR L3 sequences from a donor rabbit immunobinder into a human light chain acceptor framework into a light chain acceptor framework that has at least 85% identity to SEQ ID NO:
- 2, and
- (c) substituting framework residues in one or both of the human heavy chain acceptor framework and the human light chain framework with framework residues of the donor rabbit immunobinder.
3 more elements
Dependencies are inferred from the claim text.