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US 9,005,922 B2Granted
Sensor chip for screening topoisomerase inhibitor and screening method thereof
AssigneeTaipei Medical University
InventorsChun-Mao Lin, Hsiang-Ping Tsai, Chwen-Ming Shih, Jau-Lang Hwang, Chi-Ming Lee
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Abstract
The present invention discloses a sensor chip for screening a topoisomerase inhibitor and a screening method thereof. The sensor chip comprises topoisomerase I and a biochip. The topoisomerase I is immobilized on the biochip, and the topoisomerase I has DNA catalytic activity. This provides a rapid screening method for topoisomerase I inhibitors.
Claims7
A screening method for a topoisomerase inhibitor, comprising the following steps:
- immobilizing a topoisomerase, on a micro-fluid chip, by flowing through a solution comprising the topoisomerase with a flow rate of 10-40 μL/min for 60-300 seconds, wherein a pH value of the solution ranges from 3.0-10.0;
- adding deoxyribonucleic acid and an inhibitor to be analyzed onto the micro-fluid chip comprising the immobilized topoisomerase;
- and
- detecting a binding activity of the inhibitor to be analyzed and the immobilized topoisomerase by a surface plasmon resonance assay.
1 more element
Dependencies are inferred from the claim text.