Systems and methods for manufacturing liposomes

A method of producing a lipid vesicle encapsulating a therapeutic product, said method comprising:

• providing an aqueous solution in a first reservoir;
• providing an organic lipid solution in a second reservoir, wherein one of the aqueous solution and the organic lipid solution includes a therapeutic product;
• mixing said aqueous solution with said organic lipid solution in a first mixing region to produce a lipid vesicle solution, wherein said organic lipid solution mixes with said aqueous solution so as to substantially instantaneously produce a lipid vesicle encapsulating the therapeutic product, wherein mixing includes introducing the aqueous solution and the organic lipid solution into the first mixing region at substantially equal flow rates, wherein the first mixing region includes a first T-connector, and wherein the aqueous solution and the organic lipid solution are introduced into the first T-connector as opposing flows at substantially 180° relative to each other;
• and
• mixing said lipid vesicle solution with a buffer solution to produce a diluted lipid vesicle solution, wherein mixing said lipid vesicle solution with the buffer solution includes mixing in a second mixing region, wherein the lipid vesicle solution formed in the first mixing region is immediately and directly diluted with the buffer solution in the second mixing region less than two seconds after the lipid vesicle solution is formed, wherein the second mixing region includes a second T-connector, wherein the lipid vesicle solution and the buffer solution are introduced into the second T-connector at about 27° to about 180° relative to each other, and wherein the lipid vesicle is less than 100 nm in diameter.