Stem cell culture method
AssigneeKeele University
InventorsDeepak Kumar, Ying Yang, Nicholas Forsyth
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Abstract
Methods for culturing pluripotent stem cells on fiber scaffolds are provided which result in the expansion of the number of stem cells without loss of pluripotency. Cells obtained by such methods, implants containing such cells and medical methods using such cells are also disclosed.
Claims17
A method of culturing human embryonic stem cells (hESC) the method comprising seeding hESC onto an uncoated fiber scaffold in which the fibers are not coated or treated with, and do not incorporate, an extracellular matrix (ECM) component, and wherein the fibers have a diameter of less than about 500 nm, and incubating the hESC loaded fiber scaffold in a gas atmosphere in which the percentage of oxygen is in the range 0.25% to 15%, wherein the cultured hESC maintain stemness.
Dependencies are inferred from the claim text.