Aligned and electrospun piezoelectric polymer fiber assembly and scaffold

A three dimensional scaffolding system configured to transmit stimuli through fibers comprising:

• a first layer of aligned electrospun piezoelectric polymer fibers provided on a first substrate film;
• a second layer of aligned electrospun piezoelectric polymer fibers provided on a second substrate film;
• and
• an electrical stimulation system configured to selectively apply electrical stimulation to the first layer of fibers independently of the second layer of fibers, comprising:
• a first electrode and a second electrode disposed on the first substrate film configured to independently apply electrical stimulation to the first layer by enabling the conversion of the applied electrical stimulation to a mechanical strain on the fibers of the first layer on a molecular level without eliciting a mechanical response at a macroscale level;
• and
• a third electrode and a fourth electrode disposed on the second substrate film configured to independently apply electrical stimulation to the second layer by enabling the conversion of the applied electrical stimulation to a mechanical strain on the fibers of the second layer on a molecular level without eliciting a mechanical response at a macroscale level.

An assembly configured to utilize stimuli through bioactive fibers comprising:

• a first substrate film;
• a first electrode and a second electrode disposed on said first substrate film;
• and
• a non-woven layer of aligned, electrospun piezoelectric polymer fibers having:
• a first outer periphery region configured to be connected to said first electrode;
• a second outer periphery region configured to be connected to the second electrode;
• and
• an inner region that is suspended over a bottom surface between the first electrode and second electrode.