US 8,999,713 B2Granted

Methods of multiplying conifer embryogenic tissue

AssigneeWeyerhaeuser NR Company

InventorsPramod K. Gupta, Diane G. Holmstrom, Pamela L. Carlson

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Abstract

The invention provides methods for multiplying conifer embryogenic tissue. The methods of the invention comprise the steps of: (a) culturing conifer embryogenic tissue in or on a solid multiplication medium comprising one or more growth hormones; and (b) culturing the conifer embryogenic tissue multiplied in step (a) in or on a liquid multiplication medium comprising one or more growth hormones, wherein the initial concentration of growth hormones in the solid multiplication medium of step (a) is greater than the initial concentration of growth hormones in the liquid multiplication medium of step (b).

Claims19

Dependent claims

A method of multiplying conifer embryogenic tissue comprising the steps of:

(a) culturing conifer embryogenic tissue in or on a first multiplication medium, wherein the first multiplication medium is a solid multiplication medium comprising one or more growth hormones, to multiply the conifer embryogenic tissue;
and
(b) culturing the conifer embryogenic tissue multiplied in step (a) in or on a second multiplication medium, wherein the second multiplication medium is a liquid multiplication medium comprising one or more growth hormones, to further multiply the conifer embryogenic tissue,
wherein the initial concentration of the growth hormones in the solid multiplication medium of step (a) is about two to about three times the initial concentration of the growth hormones in the liquid multiplication medium of step (b).

1 more element

Dependencies are inferred from the claim text.

Facts

Application: 13/602,983Filed 4 Sep 2012 · us provisional application
Priority: 28 Sep 2011
Published: US 2013/0078722 A128 Mar 2013
Granted: 7 Apr 2015
Term ends: Not estimatedThe source records don’t support an estimate.

Classification4 codes

A01H 4/005
Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor: methods for micropropagation; Vegetative plant propagation…
C12N 5/04
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor: plant cells…
A01H
New plants or non-transgenic processes for obtaining them
C12N
Microorganisms or enzymes

Family

Application61/540,429Us provisional application · filed 28 Sep 2011
US 2013/0078722 A1Pre-grant publication · published 28 Mar 2013
US 8,999,713 B2This patent

From the US filing history. Foreign family members aren’t listed.

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