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US 8,961,995 B2Granted
Methods and compositions for alphavirus replicons
AssigneeThe UAB Research Foundation
InventorsIlya Frolov, Elena Frolova
Life of this patent
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Abstract
The present invention provides alphavirus replicons and methods of their use in producing heterologous protein.
Claims19
A recombinant replicon nucleic acid comprising:
- a) a nucleic acid sequence encoding a first alphavirus 5′ untranslated region (5′ UTR) and a 51 nucleotide conserved sequence element (51 -nt CSE);
- b) a nucleic acid sequence encoding alphavirus nonstructural proteins nsP1, nsP2, nsP3 and nsP4;
- c) a nucleic acid sequence encoding an alphavirus subgenornic promoter;
- d) a nucleic acid sequence encoding a second alphavirus 5′ untranslated region (5′ UTR) and 51 nucleotide conserved sequence element (51 -nt CSE);
- e) a nucleic acid sequence encoding a protease;
- f) a nucleic acid sequence encoding a heterologous protein;
- and
- g) a nucleic acid sequence encoding an alphavirus 3′ untranslated region (3′ UTR).
5 more elements
A recombinant replicon nucleic acid comprising:
- a) a nucleic acid sequence encoding a first aiphavirus 5 untranslated region (5′ UTR) and a 51 nucleotide conserved sequence element (51 -nt CSE);
- b) a nucleic acid sequence encoding alphavirus nonstructural proteins nsP1, nsP2;
- ns P3 and nsP4;
- c) a nucleic acid sequence encoding a first alphavirus subgenomic promoter;
- d) a nucleic acid sequence encoding a second alphavirus 5′ untranslated region (5′ UTR) and a 51 nucleotide conserved sequence element (51 -nt CSE);
- e) a nucleic acid sequence encoding a second alphavirus subgenomic promoter;
- f) a nucleic acid sequence encoding a heterologous protein;
- and
- g) a nucleic, acid sequence encoding an alphavirus 3′ untranslated region (3′ UTR).
6 more elements
A recombinant replicon nucleic acid comprising:
- a) a nucleic acid sequence encoding a first alphavirus 5′ untranslated region (5′ UTR) and a first 51 nucleotide conserved sequence element (51 -nt CSE);
- b) a nucleic acid sequence encoding alphavirus nonstructural proteins nsP1, nsP2, nsP3 and nsP4;
- c) a nucleic acid sequence encoding a first alphavirus subgenomic promoter;
- d) a nucleic acid sequence encoding a second alphavirus 5′ untranslated region (5′ UTR) or a modified 5′ UTR comprising a cellular Asp tRNA sequence, and a second 51 nucleotide conserved sequence element (51 -nt CSE);
- e) a nucleic acid sequence encoding a second alphavirus subgenomic promoter;
- f) a nucleic acid sequence encoding a translational enhancer derived from an Old World alphavirus;
- g) a nucleic acid sequence encoding a protease;
- h) a nucleic acid sequence encoding a heterologous protein;
- and
- i) a nucleic acid sequence encoding an alphavirus 3′ untranslated region (3′ UTR).
7 more elements
Dependencies are inferred from the claim text.