Avian adenoassociated virus and uses thereof
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsIoannis Bossis, John A. Chiorini
Life of this patent
Estimating the term…
Abstract
The present invention provides an Avian adeno-associated virus (AAAV) virus and vectors and particles derived therefrom. In addition, the present invention provides methods of delivering a nucleic acid to a cell using the AAAV vectors and particles. Methods of isolating the AAAV are provided.
Claims21
A nucleic acid vector comprising:
- a) a pair of inverted terminal repeats (ITRs), each of which is capable of forming a T-shaped hairpin structure, and wherein at least one inverted terminal repeat (ITR) comprises SEQ ID NO:20 and SEQ ID NO:21;
- and,
- b) a heterologous nucleic acid sequence between the inverted terminal repeats,
- wherein at least one ITR can be used as an origin of replication.
A recombinant AAAV virion comprising
- a) a pair of inverted terminal repeats (ITRs), each of which is capable of forming a T-shaped hairpin structure, and wherein at least one inverted terminal repeat comprises SEQ ID NO:20 and SEQ ID NO:21;
- and,
- b) a heterologous nucleic acid sequence between the inverted terminal repeats,
- wherein at least one ITR can be used as an origin of replication.
A vector comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:10, SEQ ID NO:12 and SEQ ID NO:14, wherein the vector is a plasmid, a yeast artificial chromosome or a non-AAV viral vector.
A vector that encodes a protein comprising SEQ ID NO:11, SEQ ID NO:
- 13 or SEQ ID NO:
- 15, wherein the vector is a plasmid, a yeast artificial chromosome or a non-AAV viral vector.
A vector system for producing infectious AAAV particles, said vector system comprising at least one nucleic acid molecule selected from the group consisting of:
- (a) a nucleic acid molecule comprising a pair of inverted terminal repeats (ITRs), each of which is capable of forming a T-shaped hairpin structure, and a heterologous nucleic acid sequence between the inverted terminal repeats, wherein at least one inverted terminal repeat comprises SEQ ID NO:20 and SEQ ID NO:21, and wherein at least one ITR can be used as an origin of replication;
- and,
- (b) a recombinant nucleic acid molecule that encodes a protein comprising SEQ ID NO:11, SEQ ID NO:13 or SEQ ID NO:15, wherein the recombinant nucleic acid molecule is a plasmid, a yeast artificial chromosome or a non-AAV vector.
A nucleic acid vector comprising:
- (a) a nucleic acid sequence comprising a pair of inverted terminal repeats (ITRs) flanking a heterologous nucleic acid sequence, each inverted terminal repeat being capable of forming a T-shaped hairpin structure, wherein at least one inverted terminal repeat comprises SEQ ID NO:20 and SEQ ID NO:21, and wherein at least one ITR can be used as an origin of replication;
- (b) a nucleic acid sequence encoding a protein comprising SEQ ID NO:11, SEQ ID NO:13 or SEQ ID NO:15;
- and
- (c) a nucleic acid sequence encoding a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7 and SEQ ID NO:9.
Dependencies are inferred from the claim text.