Methods for feature analysis on consecutive tissue sections

A method for feature analysis on consecutive tissue sections, comprising:

• obtaining a tissue specimen for analysis;
• sectioning said tissue specimen into at least three consecutive tissue sections comprising a first tissue section, a second tissue section, and a third tissue section, said second tissue section originating from a volume of said tissue specimen between said first and third tissue sections;
• applying at least a first biomarker to the first tissue section of said consecutive tissue sections, applying at least a second biomarker to the second tissue section, and applying at least a third biomarker to the third tissue section;
• using a digital image analysis system comprising a camera and a display screen to capture at least a first digital image of the first tissue section, a second digital image of the second tissue section and a third digital image of the third tissue section;
• and
• registering the digital images of the first and third tissue sections with the digital image of said second tissue section to digitally align each of the images;
• characterized in that the method further comprises:
• annotating one or more regions of interest within the second digital image of the second tissue section;
• transferring said one or more annotated regions of interest from the second digital image to at least one of the first and third digital images that are digitally aligned therewith;
• and
• deriving a tissue analysis score for each of said consecutive tissue sections.