US 8,785,727 B2 · Desaturase and method for the production of polyunsaturated fatty acids in transgenic organisms

Abstract

The present invention relates to a polynucleotide from Emiliana huxleyi which codes for a desaturase and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotide according to the invention, and to the polypeptides encoded by the polynucleotide. The invention furthermore relates to antibodies against the polypeptide according to the invention. Finally, the invention also relates to production methods for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.

Claims15

Dependent claims

A vector comprising a polynucleotide comprising a nucleic acid sequence selected from the group consisting of:

(a) a nucleic acid sequence that has at least 95% identity to the sequence of SEQ ID NO:
1 and
(b) a nucleic acid sequence which codes for a polypeptide having at least 90% identity to the amino acid sequence of SEQ ID NO:
2;
wherein the nucleic acid sequences of (a) and (b) code for a polypeptide with desaturase activity.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

12/990,492Filed 29 Apr 2009

Priority

EP 0815546130 Apr 2008

Published

US 2011/0138490 A19 Jun 2011

Granted

22 Jul 2014

Term ends

Not estimatedThe source records don’t support an estimate.

Classification8 codes

C12N 15/8247
Involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine: involving modified lipid metabolism, e.g. seed…
C12N 9/0083
Acting on paired donors with incorporation of molecular oxygen: miscellaneous
C12P 7/6432
Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone: eicosapentaenoic acids [EPA]
C12P 7/6434
Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone: docosahexenoic acids [DHA]

Desaturase and method for the production of polyunsaturated fatty acids in transgenic organisms

Life of this patent

Desaturase and method for the production of polyunsaturated fatty acids in transgenic organisms

Life of this patent

Abstract

Claims15