Dominant gene suppression transgenes and methods of using same
AssigneePioneer Hi-Bred International, Inc.
InventorsAndrew Mark Cigan, Erica Unger
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Abstract
Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants, and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are regulatory elements for pollen-preferred expression of linked polynucleotides. Also provided are methods for identifying gene function, methods for testing allelic variants, and methods for repressing transmission of transgenes.
Claims8
A method for selectively expressing a first polynucleotide of interest in progeny of a plant, comprising:
- (a) transforming said plant with a first construct comprising said polynucleotide operably linked to a first exogenous promoter, wherein said first plant further comprises a third construct comprising a pIR targeting a second exogenous promoter;
- (b) transforming a second plant with a second construct comprising a promoter inverted repeat (pIR) targeting said first exogenous promoter, wherein said second plant further comprises a fourth construct comprising a second polynucleotide of interest operably linked to said second exogenous promoter;
- (c) crossing said first and second plants;
- and
- (1) selecting progeny comprising said first and second constructs, where expression of said first polynucleotide of interest is not desired;
- and/or
- (2) selecting progeny comprising said first construct but not said second construct, where expression of said first polynucleotide of interest is desired.
Dependencies are inferred from the claim text.