Single molecule sensitive probes for detecting RNA
AssigneeGeorgia Tech Research Corporation
InventorPhilip J. Santangelo
Life of this patent
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Abstract
The various embodiments of the present disclosure relate generally to single molecule sensitive probes for detecting RNA, and more particularly to multivalent fluorescent probes for detecting a single molecule of RNA in a cell. The present invention includes a RNA imaging probe comprising: a multivalent core comprising a plurality of attachment sites; a plurality of RNA/DNA chimeric oligonucleotides having a specificity for a target RNA, wherein a RNA/DNA chimeric oligonucleotide is bound to an attachment site of the multivalent core; and a plurality of fluorophores, wherein a fluorophore is bound to the RNA/DNA chimeric oligonucleotide.
Claims32
A biomolecule probe comprising:
- a multivalent core comprising a plurality of attachment sites;
- a plurality of RNA/DNA chimeric linear oligonucleotides having specificity for a target, wherein the plurality of RNA/DNA chimeric linear oligonucleotides are each bound to an attachment site of the multivalent core;
- and
- a plurality of reporter molecules, wherein each reporter molecule is bound to the RNA/DNA chimeric linear oligonucleotide.
A RNA imaging probe comprising:
- a multivalent core comprising a plurality of attachment sites;
- a plurality of RNA/DNA chimeric linear oligonucleotides having a specificity for a plurality of sites on a target RNA, wherein a RNA/DNA chimeric linear oligonucleotide is bound to an attachment site of the multivalent core;
- and
- a plurality of fluorophores, wherein a fluorophore is bound to the RNA/DNA chimeric linear oligonucleotide.
Dependencies are inferred from the claim text.