Compositions and methods related to mRNA translational enhancer elements
AssigneeThe Scripps Research Institute
InventorsVincent P. Mauro, Gerald M. Edelman, Wei Zhou
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Abstract
Provided are mRNA translational enhancer elements (TEEs), e.g., SEQ ID NOs:1-35. Also provided are translational enhancer polynucleotides that comprise one or more of the specific TEEs exemplified herein or their variants, homologs or functional derivatives. Further provided are expression vectors comprising such TEEs or translational enhancer polynucleotides, as well as host cells and expression systems that harbor such vectors.
Claims14
An isolated polynucleotide sequence comprising at least two mRNA translational enhancer elements (TEE), wherein at least one TEE consists of a full-length sequence that is selected from the group consisting of 5′-CGCGGCTGA-3′ (SEQ ID NO:
- 31), 5′-AGCCGCCGCA-3′ (SEQ ID NO:
- 34) and 5′-ACGCCGCCGA-3′ (SEQ ID NO:
- 35).
A vector for recombinantly expressing a polypeptide in a eukaryotic cell, comprising a eukaryotic promoter operably linked to a polynucleotide sequence comprising at least one mRNA translational enhancer element (TEE), wherein the TEE is selected from the group consisting of 5′-CGCGGCTGA-3′ (SEQ ID NO:
- 31), 5′-AGCCGCCGCA-3′ (SEQ ID NO:
- 34) and 5′-ACGCCGCCGA-3′ (SEQ ID NO:
- 35).
A method for recombinantly producing a polypeptide comprising:
- (i) constructing an expression vector which comprises a eukaryotic promoter and a mRNA translational enhancer element that are each operably linked to a polynucleotide encoding the polypeptide, wherein the TEE is selected from the group consisting of 5′-CGCGGCTGA-3′ (SEQ ID NO:
- 31), 5′-AGCCGCCGCA-3′ (SEQ ID NO:
- 34) and 5′-ACGCCGCCGA-3′ (SEQ ID NO:
- 35);
- (ii) transfecting the expression vector into a eukaryotic host cell;
- and
- (iii) culturing the host cell transfected with the expression vector;
- thereby producing the polypeptide.
Dependencies are inferred from the claim text.