Method of preparing carrier to separate nucleic acids, carrier and micro channel to separate nucleic acids, and method and apparatus for separating nucleic acids

A method for performing nucleic acid separation, the method comprising:

• providing a microchip having a microfluid channel running therethrough;
• providing a plurality of porous microbeads within the microfluid channel, each porous microbead having at least one cation-exchangeable group bonded to at least one capturing chain on a surface of the porous microbead, the at least one cation-exchangeable group comprising a sulfoethyl group, the at least one capturing chain having a nucleic acid base sequence complementary to a target nucleic acid chain sequence, the at least one capturing chain modified with a functional group comprising an amino group;
• and
• passing at least one target nucleic acid chain corresponding to the target nucleic acid chain sequence through the porous microbead resulting in hybridization of the at least one capturing chain and the at least one target nucleic acid chain.

A method for performing nucleic acid separation, the method comprising:

• providing a microchip having a microfluid channel running therethrough;
• providing a plurality of porous microbeads within the microfluid channel, each porous microbead having a plurality of through pores adapted to permit a solution to pass rapidly therethrough and a plurality of diffusive pores adapted to permit a solute of the solution to diffuse into the plurality of diffusive pores, the porous microbead having at least one capturing chain attached to a surface of the porous microbead, the at least one capturing chain having a nucleic acid base sequence complementary to a target nucleic acid chain sequence;
• and
• passing at least one target nucleic acid chain corresponding to the target nucleic acid chain sequence through the porous microbead resulting in hybridization of the at least one capturing chain and the at least one target nucleic acid chain.