Back to results
US 8,785,211 B2Granted
Methods using pores
AssigneeIsis Innovation Limited
InventorsHagan Bayley, Yann Astier, Orit Braha
Life of this patent
Estimating the term…
Abstract
The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.
Claims12
A method of distinguishing an individual monophosphate nucleotide, comprising:
- (a) contacting the monophosphate nucleotide with a transmembrane protein pore so that the monophosphate nucleotide interacts with the pore, wherein the pore comprises a molecular adaptor that facilitates the interaction between the monophosphate nucleotide and the pore, wherein the molecular adaptor is a cyclodextrin;
- and
- (b) measuring a current passing through the pore during the interaction and thereby distinguishing the monophosphate nucleotide from other monophosphate nucleotides.
A method of sequencing a target nucleic acid sequence, comprising:
- (a) digesting an individual monophosphate nucleotide from one end of the target sequence using a processive exonuclease;
- (b) contacting the monophosphate nucleotide with a transmembrane protein pore so that the monophosphate nucleotide interacts with the pore, wherein the pore comprises a molecular adaptor that facilitates the interaction between the monophosphate nucleotide and the pore, wherein the molecular adaptor is a cyclodextrin;
- (c) measuring a current passing through the pore during the interaction and thereby determining the identity of the individual monophosphate nucleotide;
- and
- (d) repeating steps (a) to (c) at the same end of the target sequence and thereby determining the sequence of the nucleic acid.
2 more elements
Dependencies are inferred from the claim text.