Detection of nicotine metabolites
AssigneeGFC Diagnostics Ltd.
InventorsCharles John Knill, John Frederick Kennedy
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Abstract
The present invention relates to assays for detection of nicotine metabolites, in particular cotinine, in fluid samples and uses of these assays in quantification of smoking habits. The assays comprise contacting a body fluid sample with a cyanogen halide and a pyrazolone compound and detecting a change in light absorbance of the pyrazolone compound which is associated with the presence of nicotine metabolites. Also provided are assay kits including a cyanogen halide or cyanogen halide-precursor(s) and a pyrazolone compound.
Claims8
A method of detecting or measuring a nicotine metabolite in a sample comprising contacting the sample with a cyanogen halide and a pyrazolone compound and detecting a change in light absorbance of the pyrazolone compound;
- wherein the pyrazolone compound is a compound according to formula I
- in which:
- R1 is —C(═O)—;
- R2 is —CH2—;
- R3 is —C(R6)—;
- R5 is phenyl substituted with —S(═O)2OH;
- R6 is C1-6alkyl;
- z is a single bond and a is 1;
- and
- y is a double bond and b is 0.
Dependencies are inferred from the claim text.