Protein detection reagents and methods with dyes and dextrins
AssigneeExpedeon Limited
InventorsDaniel Brian Jones, Heikki Lanckriet
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Abstract
The invention provides reagents, methods and kits for detection of proteins and quantitative determination of protein concentration. The reagents comprise a protein-complexing dye, such as a Coomassie dye and one or more dextrins, for the elimination of interference caused by detergents.
Claims34
A method of detecting and/or quantifying protein comprising contacting a protein-containing sample with a solution comprising:
- (a) a protein complexing dye;
- and
- (b) one dextrin selected from α-cyclodextrins, γ-cyclodextrins, linear dextrins and derivatives thereof, or two or more dextrins;
- and
- detecting and/or quantifying dye/protein complex formation, wherein the protein is in the presence of a detergent.
A method comprising contacting a protein-containing sample in the presence of a detergent with a protein binding dye, and one dextrin selected from α-cyclodextrins, γ-cyclodextrins, linear dextrins and derivatives thereof;
- and detecting and/or quantifying dye/protein complex formation, wherein the protein is in the presence of a detergent.
A method of detecting and/or quantifying protein comprising contacting a protein-containing sample in the presence of a detergent with a protein binding dye, and two or more dextrins;
- and detecting and/or quantifying dye/protein complex.
A method of detecting and/or quantifying protein comprising contacting a protein-containing sample with a solution comprising:
- (a) a protein-complexing dye, wherein the dye is a Coomassie dye;
- and
- two or more dextrins;
- and
- detecting and/or quantifying dye/protein complex formation, wherein the protein is on a support.
A method of detecting and/or quantifying protein comprising contacting a protein-containing sample with a solution comprising:
- (a) a protein-complexing dye;
- and
- (b) one dextrin selected from α-cyclodextrins, γ-cyclodextrins, linear dextrins and derivatives thereof;
- and
- detecting and/or quantifying dye/protein complex formation, wherein the protein is on a support.
Dependencies are inferred from the claim text.