Culture medium and method for inducing differentiation into adipocytes
AssigneeFujirebio Inc.
InventorYoshiyuki Hotta
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AssigneeFujirebio Inc.
InventorYoshiyuki Hotta
Estimating the term…
Disclosed are a culture medium, an additive, and a method for efficiently inducing the differentiation of mammalian somatic stem cells into cells having the characteristics of adipocytes under conditions of serum-free or low-serum culture medium. The culture medium for inducing the differentiation of mammalian somatic stem cells into adipocytes comprises a basal medium for culturing mammalian cells, an agent for inducing the differentiation of mammalian somatic stem cells into adipocytes, biotin, a ligand for endothelial cell differentiation gene (Edg) family receptors, vitamin C, and HEPES, which culture medium is serum-free or contains a low concentration of serum.
A culture medium for inducing differentiation of mammalian somatic stem cells into adipocytes, comprising a basal medium for culturing mammalian cells, an agent for inducing the differentiation of mammalian somatic stem cells into adipocytes, biotin, a ligand for endothelial cell differentiation gene (Edg) family receptors, vitamin C, and HEPES, which culture medium is serum-free.
An additive to a culture medium for inducing the differentiation of mammalian somatic stem cells into adipocytes, the additive comprising biotin, a ligand for endothelial cell differentiation gene (Edg) family receptors, vitamin C, and HEPES.
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