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AssigneeLife Technologies Corporation
InventorsStephen Gorfien, Richard Fike, Glenn Godwin, Joyce Dzimian, David A. Epstein, Dale Gruber, Don McClure, Paul Price
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The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
A suspension cell culture comprising a serum-free and protein-free cell culture medium and a Chinese Hamster Ovary (CHO) cell, wherein said culture medium comprises a transferrin substitute and an insulin substitute, said transferrin substitute comprising an iron or an iron containing compound and said insulin substitute comprising a zinc or a zinc containing compound, wherein said culture medium supports increased growth of the CHO cell in suspension as compared to cultivation in FMX-8 medium without supplementation of serum, and without supplementation of protein, and without expression of an exogenous recombinant protein in the CHO cell.
Dependencies are inferred from the claim text.