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US 8,785,176 B2Granted
Post protein hydrolysis removal of a potent ribonuclease inhibitor and the enzymatic capture of DNA
AssigneeUniversity of South Florida
InventorsMatt Ewert, Philip Amuso, Andrew Cannons
Life of this patent
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Abstract
The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample.
Claims13
A method for capturing a particle from a biological sample including the particle, the method comprising:
- (a) contacting the biological sample with:
- (i) 2 to 10 U plasminogen and 5,000 to 10,000 U streptokinase or plasmin produced by combining 2 to 10 U plasminogen and 5,000 to 10,000 U streptokinase,
- (ii) 200 to 3,650 U DNase,
- (iii) 1,500 to 4,500 KU phospholipase A2, and
- (iv) a detergent;
- and
- (b) capturing the particle,
- wherein the particle is a prion, a toxin, a metabolic marker, cancerous matter, a disease state marker, a bacterium, a virus, or a fungus.
5 more elements
Dependencies are inferred from the claim text.