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US 8,785,170 B2Granted
Variant CBH2 cellulases and related polynucleotides
AssigneeCodexis, Inc.
InventorsIsh K. Dhawan, Erika N. Segraves
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Abstract
The invention provides variants of a Streptomyces sp. CBH2 that have improved properties compared to the wild type enzyme and methods of using the variants in the hydrolysis of substrates comprising cellulose.
Claims23
An isolated Streptomyces sp cellobiohydrolase II (CBH2) polypeptide variant comprising:
- an amino acid sequence that is at least 90% identical to the wild type Streptomyces sp CBH2 of SEQ ID NO:-4 and comprises the substitution L201F, wherein the amino acid position is determined by optimal alignment with SEQ ID NO:-4, and wherein said CBH2 variant exhibits at least 2-fold improvement in cellulase activity compared to the wild-type Streptomyces sp. CBH2 set forth in SEQ ID NO:4.
Dependencies are inferred from the claim text.