Single pH process for starch liquefaction and saccharification for high-density glucose syrups
AssigneeDanisco US Inc.
InventorsSung Ho Lee, Jayarama K. Shetty, Paula Johanna Maria Teunissen
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Abstract
Embodiments of the present disclosure relate to a process for producing downstream products, such as high-density glucose syrups and high-glucose fermentation feedstock, from a starch-containing substrate without a pH adjustment before the saccharification step. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.2 to 5.6.
Claims19
A method of processing a starch comprising saccharification of a starch substrate to glucose in the presence of a glucoamylase without a pH adjustment, wherein saccharification is performed at pH 5.2 to 5.6 and at a temperature in the range of about 58° C. to about 62° C., wherein the glucoamylase is selected from the group consisting of a parent Humicola grisea glucoamylase (HgGA) comprising SEQ ID NO:
- 3 and a variant thereof, wherein the variant has at least 99% identity to the parent glucoamylase.
Dependencies are inferred from the claim text.