Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein
AssigneeE. I. Du Pont de Nemours and Company
InventorsSeung-Pyo Hong, Quinn Qun Zhu
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Abstract
Recombinant oleaginous microorganisms having increased oil content due to the expression of a caleosin polypeptide are described. A recombinant oleaginous microorganism of the disclosed invention produces at least 25% of its dry cell weight as oil, and comprises a functional polyunsaturated fatty acid (PUFA) biosynthetic pathway and at least one genetic construct encoding a caleosin polypeptide. A method for increasing the amount of oil in a recombinant oleaginous microorganism is also described.
Claims7
A recombinant oleaginous Yarrowia lipolytica that produces at least 25% of its dry cell weight as oil, wherein said recombinant oleaginous Yarrowia lipolytica comprises a functional polyunsaturated fatty acid biosynthetic pathway and at least one genetic construct encoding a caleosin polypeptide comprising the amino acid sequence of SEQ ID NO:2;
- further wherein said recombinant oleaginous Yarrowia lipolytica comprises at least 35% eicosapentaenoic acid as a weight percentage of total fatty acids of the Yarrowia lipolytica.
Dependencies are inferred from the claim text.