Extracellular secretion of recombinant proteins
AssigneeAlliance for Sustainable Energy, LLC
InventorsJeffrey G. Linger, Aldis Darzins
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Abstract
Nucleic acids encoding secretion signals, expression vectors containing the nucleic acids, and host cells containing the expression vectors are disclosed. Also disclosed are polypeptides that contain the secretion signals and methods of producing polypeptides, including methods of directing the extracellular secretion of the polypeptides. Exemplary embodiments include cellulase proteins fused to secretion signals, methods to produce and isolate these polypeptides, and methods to degrade lignocellulosic biomass.
Claims18
A recombinant vector comprising an isolated nucleic acid molecule encoding a polypeptide that functions as a secretion signal in Zymomonas mobilis, wherein the polypeptide has an amino acid sequence at least 98% identical to the amino acid sequence of SEQ ID NO:2.
Dependencies are inferred from the claim text.