Method for quantitative measurements of HDL-C and LDL-C
AssigneeBeijing Strong Biotechnologies, Inc.
InventorGuojing Sun
Life of this patent
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Abstract
The present invention relates to a method for direct measurement of high-density lipoprotein cholesterol. (HDL-C) or low-density lipoprotein cholesterol (LDL-C) based on a modified chemical precipitation method, wherein the improvements were made by optimizing the concentrations and proportions of potassium polyvinyl sulfate (PVSK) and polyethylene glycol methyl ether (PEGME) in such a way that the enzymatic reactions are completed before precipitation formations. The method in combination with certain surfactants showed good correlations with other direct methods used for HDL-C or LDL-C determinations.
Claims11
A method for measuring the contents of high-density lipoprotein cholesterol in a sample, comprising the steps of:
- 1) contacting potassium polyvinyl sulfate and polyethylene glycol methyl ether with the sample, said potassium polyvinyl sulfate and polyethylene glycol methyl ether being in an optimized proportion and having optimized concentrations wherein said optimized proportion between potassium polyvinyl sulfate and polyethylene glycol methyl ether is 1:500-550, and said optimized concentrations thereof are 10-20 mg/L and 5 g-11 g/L, respectively for measurement of high-density lipoprotein cholesterol;
- 2) measuring the concentrations of high-density lipoprotein cholesterol in the sample enzymatically in combination with at least one surfactant before the formation of precipitation.
A method for measuring the contents of low-density lipoprotein cholesterol in a sample, comprising the steps of:
- 1) contacting potassium polyvinyl sulfate and polyethylene glycol methyl ether with the sample, said potassium polyvinyl sulfate and polyethylene glycol methyl ether being in an optimized proportion and having optimized concentrations wherein said optimized proportion between potassium polyvinyl sulfate and polyethylene glycol methyl ether is 1:400-500, and said optimized concentrations thereof are 10-20 mg/L and 4 g-10 g/L, respectively, for measurement of low-density lipoprotein cholesterol;
- 2) measuring the concentrations of low-density lipoprotein cholesterol in the sample enzymatically in combination with at least one surfactant before the formation of precipitation.
Dependencies are inferred from the claim text.