Method for immobilizing streptavidin on a self-assembled monolayer
AssigneePanasonic Healthcare Co., Ltd.
InventorYukari Hataoka
Life of this patent
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Abstract
Provided are a method for increasing an amount of streptavidin to be immobilized on the self-assembled monolayer and a sensor which comprises streptavidin immobilized with the method. The method of the current technology is characterized by that one molecule of an amino acid is interposed between the self-assembled monolayer and the molecule of streptavidin.
Claims12
A method for immobilizing a streptavidin on a self-assembled monolayer, comprising the following step (a) and step (b) in this order:
- a step (a) of preparing a substrate comprising one molecule of an amino acid and the self-assembled monolayer, wherein,
- the one molecule of the amino acid is bound to the self-assembled monolayer through a peptide bond represented by the following chemical formula (I):
- wherein R represents a side chain of the one molecule of the amino acid, and wherein the one molecule of the amino acid is selected from the group consisting of Lysine, Histidine, Phenylalanine, Cysteine, Glycine, Methionine, Glutamate, Tyrosine, Alanine, Serine, Aspartate, Asparagine, Leucine, and Tryptophan, and
- a step (b) of supplying the streptavidin to the substrate to form a peptide bond represented by the following chemical formula (II) as a result of reaction between the carboxyl group of the one molecule of the amino acid and the amino group of the streptavidin
- wherein R represents a side chain of the one molecule of the amino acid.
A method for detecting or quantifying a target substance contained in a sample with a sensor, comprising the following steps (a) to (d) in this order,
- a step (a) of preparing a sensor substrate comprising one molecule of an amino acid and a self-assembled monolayer, wherein, the one molecule of the amino acid is bound to the self-assembled monolayer through a peptide bond represented by the following chemical formula (I):
- wherein R represents a side chain of the one molecule of the amino acid, and wherein the one molecule of the amino acid is selected from the group consisting of Lysine, Histidine, Phenylalanine, Cysteine, Glycine, Methionine, Glutamate, Tyrosine, Alanine, Serine, Aspartate, Asparagine, Leucine, and Tryptophan;
- a step (b) of supplying the streptavidin to the sensor substrate to form a peptide bond represented by the following chemical formula (II) as a result of reaction between the carboxyl group of the one molecule of the amino acid and the amino group of the streptavidin
- wherein R represents a side chain of the one molecule of the amino acid,
- a step (c) of supplying the sample to the sensor substrate of formula (II) to cause the target substance to bind to the streptavidin, and
- a step (d) of detecting or quantifying the target substance.
Dependencies are inferred from the claim text.