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US 8,785,139 B2Granted
Modification-dependent activity assays
AssigneesBaxter International Inc., Baxter Healthcare S.A.
InventorsAlfred Weber, Andrea Engelmaier, Hans-Peter Schwarz
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Abstract
Disclosed herein are methods, systems and kits to measure the presence and/or activity of recombinant polypeptides comprising a modification.
Claims28
A method for detecting the presence of a recombinant polypeptide comprising a modification, the method comprising the steps of:
- incubating a sample including the recombinant polypeptide comprising the modification with a capture agent that selectively binds the modification under conditions allowing the selective binding of the capture agent to the modification, thereby forming a polypeptide-agent complex;
- purifying the polypeptide-agent complex from the sample;
- and
- assaying for the presence of the recombinant polypeptide and/or a polypeptide activity, wherein detection of the recombinant polypeptide and/or the polypeptide activity is indicative of the presence of the recombinant polypeptide comprising the modification.
1 more element
Dependencies are inferred from the claim text.