US 8,785,120 B2 · Method for the treatment of a sample containing biomolecules

Abstract

The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo-oder heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carbonxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides in particular a method for the lysis of a biological sample, and methods for the stabilization of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

Claims20

Dependent claims

A method for stabilizing at least one species of nucleic acid and species of protein comprised in a sample, said method comprising mixing the sample with a fluid preparation to form a sample preparation comprising the at least one species of nucleic acid and the at least one species of the protein, and wherein the fluid preparation comprises at least one nitrogenous compound which is at least one selected from the group consisting of:

a) polyamines;
b) amino acids, and oligo and polypeptides;
c) nitrogenous heterocyclic compounds including homo or hetero polymers which comprise at least one nitrogenous compound;
d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another and are selected from the group consisting of H, C1-C5 alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously;
e) carboxylic acid amides;
f) inorganic ammonium salts;
g) inner salt compounds containing ammonium groups;
h) antibiotics binding nucleic acid;
and
i) compounds which bind in the small cavity of DNA;
wherein an amount of the nitrogenous compound is chosen so as to minimize precipitation of the nucleic acid and protein present during and after said mixing, wherein the ratio of concentration of the at least one species of nucleic acid and protein in the sample preparation is the same as the ratio of concentration of the at least one species of nucleic acid and protein in the sample, and wherein the species of nucleic acid and species of protein in the sample are not encapsulated by a cell membrane.

8 more elements

a) polyamines;
b) amino acids, and oligo and polypeptides;
c) nitrogenous heterocyclic compounds including homo or hetero polymers which comprise at least one nitrogenous compound;
d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another and are selected from the group consisting of H, C1-C5 alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously;
e) carboxylic acid amides;
f) inorganic ammonium salts;
g) inner salt compounds containing ammonium groups;
h) antibiotics binding nucleic acid;
and
i) compounds which bind in the small cavity of DNA;
wherein an amount of the nitrogenous compound is chosen so as to minimize precipitation of the nucleic acid and protein present during and after said mixing,
wherein the ratio of concentration of the at least one species of nucleic acid and protein in the sample preparation is the same as the ratio of concentration of the at least one species of nucleic acid and protein in the sample,
wherein the species of nucleic acid and species of protein in the sample are not encapsulated by a cell membrane, and
wherein said sample comprises a biological sample which was subjected to lysis and optionally subjected to at least one further purification step, before said mixing with said fluid preparation.

11 more elements

Dependencies are inferred from the claim text.

Method for the treatment of a sample containing biomolecules

Life of this patent

Method for the treatment of a sample containing biomolecules

Life of this patent

Abstract

Claims20