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US 8,785,118 B2Granted
Method for fixing a supercoiled DNA and the use for analyzing the DNA repair
AssigneeCommissariat A L'energie Atomique Et Aux Energies Alternatives
InventorsJean-François Millau, Sylvie Sauvaigo
Life of this patent
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Abstract
A method for fixing a supercoiled DNA consists in deposing a supercoiled DNA sample on the surface of a porous polymer film, in fixing said supercoiled DNA therein by a passive diffusion, in obtaining a support by the inventive method and in using said support for analyzing the DNA distribution.
Claims21
A method for immobilizing a supercoiled DNA, which comprises the steps of:
- a) depositing the supercoiled DNA sample on a surface of a porous polymer film, and b) immobilizing the supercoiled DNA by a passive diffusion in said porous polymer film, wherein the immobilization of the supercoiled DNA comprises no additional electrophoretic or physical or chemical treatment of the porous polymer film or the supercoiled DNA.
Dependencies are inferred from the claim text.