Separation medium for capillary electrophoresis suppressing electroosmotic flow in bare capillaries and channels

A separation medium for capillary sieving electrophoresis comprising:

• a) a neutral hydrophilic polymer at the concentration from about 0 g/L to about 200 g/L;
• b) a neutral polyol at the concentration from about 20 g/L to about 200 g/L;
• c) a biopolymer denaturant at the concentration from about 0 g/L to about 420 g/L;
• d) a cationic counter ion at the concentration between about 0.02 M and about 3 M;
• e) boric acid at the concentration from about 0.1 M and about 3 M, with the proviso that
• 1) if said biopolymer denaturant is SDS and said counter ion is Tris the concentration of boric acid is in the range from about 1.1 M to about 3 M;
• 2) if said counter ion is sodium the concentration of boric acid is in the range from about 0.8 M to about 3 M.