Nanopore device and a method for nucleic acid analysis

A nanopore device, arranged to cooperate with a sample of nucleic acid, the nanopore device comprising:

• an input chamber, for containment of the sample of nucleic acid;
• a first chamber connected to the input chamber by a first nanopore for detecting a first target molecule;
• means to apply an electrical potential difference across the first nanopore, the electrical potential being arranged to draw a first fragment of the sample of nucleic acid through the first nanopore;
• and
• an electrolytic solution which is capable of flow through the first nanopore under the influence of the electrical potential difference, the flow detectable as a first ionic current, changes in the first ionic current being indicative of detection of the first target molecule wherein, the nanopore device further comprises:
• a second chamber connected to the input chamber by a second nanopore for detecting a second target molecule, the second target molecule being different from the first target molecule;
• the means to apply an electrical potential difference being arranged to apply an electrical potential difference across the second nanopore which is equal to, and common with, the electrical potential difference across the first nanopore;
• the electrical potential across the second nanopore being arranged to draw a second fragment of the sample of nucleic acid through the second nanopore and to influence the electrolytic solution to flow through the second nanopore;
• and
• the flow being detectable as a second ionic current, changes in the second ionic current being indicative of detection of a second target molecule by the second nanopore.