Ultrasonic analyte concentration and application in flow cytometry

A system, comprising:

• an inlet for accepting a fluid having particles therein;
• a substantially cylindrical chamber for flowing the fluid having particles therein;
• an acoustic radiation pressure applier configured to apply acoustic radiation pressure to the fluid, the acoustic radiation pressure being adapted to cause an effect on the particles within the fluid, and wherein the acoustic radiation pressure applier is configured to apply acoustic radiation pressure to the fluid by acoustically exciting the length of the cylindrical chamber;
• a monitoring transducer configured to monitor a frequency of the acoustic radiation pressure;
• an interrogation light source configured to illuminate the particles;
• and
• a photomultiplier tube configured collect information from the interrogated particles, the information comprising information to determine molecular makeup of the particles.

A method of imparting movement to particles in a fluid comprising:

• supplying a fluid having particles therein to a flow cytometer comprising a substantially cylindrical chamber;
• applying acoustic radiation pressure to the fluid, wherein said acoustic radiation pressure causes an effect on the particles within the fluid, and wherein applying acoustic radiation pressure to the fluid comprises acoustically exciting the length of the cylindrical chamber;
• and
• outputting a radio frequency electrical signal.

A method of analyzing particles in a fluid comprising:

• supplying a fluid having particles therein to a flow cytometer comprising a substantially cylindrical chamber;
• applying acoustic radiation pressure to the fluid, wherein said acoustic radiation pressure causes an effect on the particles within the fluid, and wherein applying acoustic radiation pressure to the fluid comprises acoustically exciting the length of the cylindrical chamber;
• analyzing the particles, wherein analyzing the particles comprises:
• subjecting the particles to an interrogation light source;
• and
• collecting information from the interrogated particles;
• and
• identifying the information to determine molecular makeup of the particles.

A method of imparting movement to particles in a fluid comprising:

• supplying a fluid having particles therein to a flow cytometer comprising a substantially cylindrical chamber;
• applying acoustic radiation pressure to the fluid, wherein said acoustic radiation pressure causes an effect on the particles within the fluid, and wherein applying acoustic radiation pressure to the fluid comprises acoustically exciting the length of the cylindrical chamber;
• adjusting the acoustic radiation pressure to maintain a resonant frequency;
• and
• collecting Raman signals from the particles.

A method of analyzing particles in a fluid comprising:

• supplying a fluid having particles therein to a flow cytometer comprising a substantially cylindrical chamber;
• applying acoustic radiation pressure to the fluid, wherein said acoustic radiation pressure causes an effect on the particles within the fluid, and wherein applying acoustic radiation pressure to the fluid comprises acoustically exciting the length of the cylindrical chamber;
• and
• collecting Raman signals from the particles.