Nucleic acids useful in the manufacture of oil
AssigneeSolazyme, Inc.
InventorsScott Franklin, Aravind Somanchi, Karen Espina, George Rudenko, Penelope Chua
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Abstract
Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.
Claims9
A host cell of the family Chlorellaceae having a native 23S rRNA with at least 90% sequence identity to SEQ ID NO. 15, comprising a recombinant nucleic acid codon-optimized for Prototheca and encoding a stearoyl-ACP desaturase, the recombinant nucleic acid containing the most preferred codon of Table 1 for at least 80% of the codons that encode the stearoyl-ACP desaturase of SEQ ID NO. 145 such that the codon-optimized nucleic acid is more efficiently expressed in the host cell than a non-codon-optimized nucleic acid thereby altering the fatty acid profile of the host cell.
Dependencies are inferred from the claim text.