Chromatography ligand comprising domain C from staphylococcus aureus protein A for antibody isolation

A process of isolating one or more target compounds, which process comprises contacting a liquid comprising said compound(s) with a chromatography matrix;

• allowing said compound(s) to adsorb to ligands present on the matrix, wherein said ligands comprise at least one of a Staphylococcus protein A (SpA) Domain C sequence comprising an amino acid sequence selected from the group consisting of SEQ ID NO 1 or SEQ ID NO:
• 2, and, optionally, eluting said compound(s) by the passing across said matrix of a liquid that releases them from ligands, wherein said ligands are coupled to a matrix/carrier and wherein said ligands further comprise a terminal coupling group, said group preferably comprising at least one nitrogen and/or sulphur atom(s), wherein said process further includes an alkaline regeneration step and is carried out at least 80 times.