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US 8,772,234 B2Granted
BMP10 propeptides and related methods
AssigneeAcceleron Pharma Inc.
InventorsJasbir Seehra, John Knopf
Life of this patent
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Abstract
In certain aspects, the present invention provides BMP10 propeptides for use in treating a variety of disorders including heart disorders and other disorders associated with unwanted activity of the mature BMP10 polypeptide. The present invention also provides methods of screening compounds that modulate activity of BMP10.
Claims10
A pharmaceutical preparation comprising:
- a) a polypeptide comprising a bone morphogenetic protein-10 (BMP10) propeptide;
- and
- b) a pharmaceutically acceptable carrier,
- wherein the BMP10 propeptide comprises an amino acid sequence at least 98% identical to the amino acid sequence of SEQ ID NO:
- 1, wherein the BMP10 propeptide contains no more than 20 contiguous amino acids of a mature portion of a BMP10 polypeptide and comprises an alteration in the amino acid sequence that alters glycosylation of the BMP10 propeptide relative to the naturally occurring BMP10 propeptide when produced in a mammalian cell, and
- wherein said BMP10 propeptide has one or more of the following characteristics:
- i) binds to mature BMP10 with a Kd of at least 10−5 M;
- and
- ii) inhibits BMP10 signaling in a cell.
6 more elements
A BMP10 propeptide fusion protein comprising:
- a BMP10 propeptide and an IgG Fc portion;
- wherein the BMP10 propeptide comprises an amino acid sequence at least 98% identical to the amino acid sequence of SEQ ID NO:
- 1, wherein the BMP10 propeptide contains no more than 20 contiguous amino acids of a mature portion of a BMP10 polypeptide and comprises an alteration in the amino acid sequence that alters glycosylation of the BMP10 propeptide relative to the naturally occurring BMP10 propeptide when produced in a mammalian cell, and
- wherein said BMP10 propeptide has one or more of the following characteristics:
- i) binds to mature BMP10 with a Kd of at least 10−5M;
- and
- ii) inhibits BMP10 signaling in a cell.
4 more elements
Dependencies are inferred from the claim text.