US 8,772,031 B2 · Composition for reprogramming somatic cells to generate induced pluripotent stem cells, comprising Oct4 in combination with Bmi1 or its upstream regulator, and method for generating induced pluripotent stem cells using the same

Abstract

Disclosed is a composition for reprogramming somatic cells to generate embryonic stem cell-like cells, comprising: a) a Bmi1 (B cell-specific Moloney murine leukemia virus integration site 1) protein or a nucleic acid molecule coding for Bmi1; and b) an Oct4 protein or a nucleic acid molecule coding for Oct4. Also, a method is provided for reprogramming somatic cells to generate embryonic stem cell-like cells using the composition. In addition to reducing the number of the genetic factors conventionally needed, the composition and method allow the generation of pluripotent embryonic stem cell-like cells which have high potential in the cell therapy of various diseases.

Claims1

Dependent claims

A method for reprogramming somatic cells to generate induced mouse pluripotent stem cells, comprising steps of:

(i) simultaneously treating mouse fibroblasts with a Shh (Sonic hedgehog signaling) protein, oxysterol, purmorphamine, and a packaging cell expressing a vector encoding Oct4, wherein the treated fibroblasts are cultured in a neural stem cell media containing DMEM/F12, B27, N2, bFGF, and EGF
(ii) culturing the cells produced from step (i) in an embryonic stem cell culture conditions including DMEM, LIF, and a feeder layer;
thereby producing induced mouse pluripotent stem cells.

Dependencies are inferred from the claim text.

Facts

Application

13/890,896Filed 9 May 2013 · division

Priority

KR 10-2009-013496630 Dec 2009

Published

US 2013/0280808 A124 Oct 2013

Granted

8 Jul 2014

Term ends

Not estimatedThe source records don’t support an estimate.

Classification24 codes

C12N 5/0606
Embryonic cells ; Embryoid bodies: pluripotent embryonic cells, e.g. embryonic stem cells [ES]
A61P 1/04
Drugs for disorders of the alimentary tract or the digestive system: for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of…
A61P 1/16
Drugs for disorders of the alimentary tract or the digestive system: for liver or gallbladder disorders, e.g. hepatoprotective agents,…
A61P 3/06
Drugs for disorders of the metabolism: antihyperlipidemics

Composition for reprogramming somatic cells to generate induced pluripotent stem cells, comprising Oct4 in combination with Bmi1 or its upstream regulator, and method for generating induced pluripotent stem cells using the same

Life of this patent

Composition for reprogramming somatic cells to generate induced pluripotent stem cells, comprising Oct4 in combination with Bmi1 or its upstream regulator, and method for generating induced pluripotent stem cells using the same

Life of this patent

Abstract

Claims1