Methods and compositions for inactivating alpha 1,6 fucosyltransferase (FUT8) gene expression
AssigneeSangamo BioSciences, Inc.
InventorsTrevor Collingwood, Gregory J. Cost
Life of this patent
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Abstract
Disclosed herein are methods and compositions for inactivating a FUT8 gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
Claims22
A zinc finger DNA-binding domain, wherein the DNA-binding domain comprises four zinc finger recognition regions ordered F1 to F4 from N-terminus to C-terminus, and wherein F1, F2, F3, and F4 comprise the following amino acid sequences:
- F1:
- QSSDLSR (SEQ ID NO:
- 9);
- F2:
- TSGNLTR (SEQ ID NO:
- 10);
- F3:
- RSDDLSK (SEQ ID NO:
- 11);
- and
- F4:
- DRSALAR (SEQ ID NO:
- 12).
A zinc finger DNA-binding domain, wherein the DNA-binding domain comprises five zinc finger recognition regions ordered F1 to F5 from N-terminus to C-terminus, and wherein F1, F2, F3, F4, and F5 comprise the following amino acid sequences:
- FI:
- RSDNLRE (SEQ ID NO:
- 26);
- F2:
- NNTQLIE (SEQ ID NO:
- 27);
- F3:
- TSSILSR (SEQ ID NO:
- 28);
- F4 RSDNLSA (SEQ ID NO:
- 29);
- and
- F5:
- RKDTRIT (SEQ ID NO:
- 30).
Dependencies are inferred from the claim text.