Media for affinity chromatography
AssigneeEMD Millipore Corporation
InventorsNanying Bian, Senthil Ramaswamy, Neil Soice, Chen Wang, Yuan Wong
Life of this patent
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Abstract
The invention relates generally to solid supports for chromatography. In specific embodiments the invention provides for solid supports suitable for affinity chromatography along with methods, systems and kits which use the same.
Claims14
A solid support suitable for performing affinity chromatography comprising
- i) a silica particle having a pore size greater than 630 Å and less than 1000 Å and a mean silica particle size greater than 50 μm and less than 70 μm, and
- ii) an affinity ligand linked to the solid support wherein the affinity ligand is selected from the group consisting of proteins, enzymes, antibodies, immunoglobulins, and protein receptors and the affinity ligand density is 12 g/L to 14 g/L.
A kit comprising an affinity chromatography solid support comprising a silica particle having a pore size greater than 630 Å and less than 1000 Å and a mean particle size greater than 55 μm and less than 70 μm, an affinity ligand linked to the solid support having an affinity ligand density from greater than 15 g/L, and a dynamic capacity for a target molecule 50 g/L to 60 g/L, wherein the affinity ligand is selected from the group consisting of proteins, enzymes, antibodies, immunoglobulins, and protein receptors, and
- and at least one container.
A solid support suitable for performing affinity chromatography comprising
- i) a silica bead selected from the group consisting of a particulate glass, controlled porous glass, colloidal silica, wollastonite, dried silica gel and bentonite, having a pore size from about 782 Å to 932 Å and a mean particle size from about 55 μm to 67 μm, and
- ii) an affinity ligand, having an affinity ligand density from 12 g/L to 14 g/L, linked to the solid support wherein the affinity ligand is selected from the group consisting of proteins, enzymes, antibodies, immunoglobulins, and protein receptors,
- wherein the affinity ligand has a dynamic capacity for a target molecule at least 50 g/L.
A kit comprising an affinity chromatography solid support and a column suitable for packing the affinity chromatography solid support,
- the affinity chromatography solid support comprises a silica particle having a pore size greater than about 782 Å to 932 Å and a mean particle size from 55 μm to 67 μm, an affinity ligand linked to the solid support having an affinity ligand density from 12 g/L to 14 g/L, and a dynamic capacity for a target molecule 50 g/L to 60 g/L, wherein the affinity ligand is selected from the group consisting of proteins, enzymes, antibodies immunoglobulins, and protein receptors, and at least one container.
Dependencies are inferred from the claim text.