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US 8,771,989 B2Granted
Vectors and yeast strains for protein production: Ca2+ ATPase overexpression
AssigneeGlycoFi, Inc.
InventorByung-Kwon Choi
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Abstract
Lower eukaryote host cells in which an endogenous or heterologous Ca2+ ATPase is overexpressed are described. Also described are lower eukaryote host cells in which a calreticulin and/or ERp57 protein are overexpressed. These host cells are useful for producing recombinant glycoproteins that have reduced O-glycosylation.
Claims7
A method for producing a recombinant antibody having reduced O-glycosylation comprising:
- (a) providing a Pichia pastoris host comprising a deletion or disruption of a β-mannosyltransferase gene and at least one phosphomannosyl transferase gene and a nucleic acid molecule encoding at least one endogenous or exogenous Ca2+ ATPase wherein expression of the Ca2+ ATPase in the host cell is ectopic;
- (b) introducing a nucleic acid molecule into the host cell encoding the recombinant antibody:
- and
- (c) growing the host cell under conditions suitable for producing the recombinant antibody.
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