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US 8,771,981 B2Granted
Method for producing an L-amino acid
AssigneeAjinomoto Co., Inc.
InventorShigeo Suzuki
Life of this patent
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Abstract
A method for producing an L-amino acid by preparing a processed product of a microalgae, which promotes production and accumulation of the L-amino acid by a bacterium having an ability to produce the L-amino acid, by culturing the microalgae in a medium, and processing the resulting culture at a midtemperature; culturing the bacterium in a medium containing the processed product of the microalgae to produce and accumulate the L-amino acid in culture; and collecting the L-amino acid from the culture.
Claims7
A method for producing an L-amino acid, which comprises:
- (a) preparing a processed product of a microalga, which promotes production and accumulation of the L-amino acid by a bacterium having an ability to produce the L-amino acid, comprising:
- (i) culturing the microalga in a first medium, followed by
- (ii) incubating the cultured microalga of step (i) at a mid-temperature of 40° C. to 70° C., and
- (iii) performing centrifugation on the product of step (ii), resulting in a precipitate and supernatant;
- and
- (b) culturing the bacterium in a second medium comprising the supernatant of step (iii) to produce and accumulate the L-amino acid in the second medium, wherein the supernatant comprises glucose or glycerol;
- and
- (c) collecting the L-amino acid from the second medium.
5 more elements
Dependencies are inferred from the claim text.