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US 8,771,977 B2Granted
Method of microbial profiling of a sample using phenanthridium derivatives
AssigneeMontana State University
InventorsAndreas Nocker, Anne K. Camper
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Abstract
The present disclosure provides for a method of microbial profiling of a sample using phenanthridium derivatives with a 2+ charge or higher.
Claims10
A method of microbial profiling of a sample, comprising:
- a. collecting a sample;
- b. exposing said sample to a phenanthridium derivative of formula (II):
- wherein
- R1 is an alkyl having 1 to 6 carbon atoms, perfluoroalkyl having 1 to 6 carbon atoms, or tetra-alkyl ammonium;
- R2 is a substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, or substituted or unsubstituted cycloalkyl having 3 to 10 carbon atoms;
- R3 is amino, N-alkylamino, N,N-dialkylamino, alkoxy, or carboxyalkyl;
- and
- R4 and R5 are independently selected from the group consisting of:
- hydrogen, halide, hydroxyl, amino, N-alkylamino, N,N-dialkylamino, alkoxy, carboxyalkyl, alkyl having 1 to 6 carbon atoms, perfluoroalkyl having 1 to 6 carbon atoms, azide, and tetra-alkyl ammonium;
- or a mono or bis halide salt thereof,
- wherein the phenanthridium derivative has an overall charge of 2+ or higher;
- c. exposing said phenanthridium derivative containing sample to a light source;
- d. optionally isolating genomic DNA from said sample;
- and
- e. performing PCR on said isolated DNA.
12 more elements
A method of microbial profiling of a sample comprising:
- a. collecting a sample;
- b. exposing said sample to a phenanthridium derivative of formula (II):
- wherein
- R1 is an alkyl having 1 to 6 carbon atoms, perfluoroalkyl having 1 to 6 carbon atoms, or tetra-alkyl ammonium;
- R2 is a substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, or substituted or unsubstituted cycloalkyl having 3 to 10 carbon atoms;
- R3 is amino, N-alkylamino, N,N-dialkylamino, alkoxy, or carboxyalkyl;
- and
- R4 and R5 are independently selected from the group consisting of:
- hydrogen, halide, hydroxyl, amino, N-alkylamino, N,N-dialkylamino, alkoxy, carboxyalkyl, alkyl having 1 to 6 carbon atoms, perfluoroalkyl having 1 to 6 carbon atoms, azide, and tetra-alkyl ammonium;
- or a mono or bis halide salt thereof,
- wherein the phenanthridium derivative has an overall charge of 2+ or higher;
- c. exposing said phenanthridium derivative containing sample to a light source;
- d. optionally isolating RNA from said sample;
- and
- e. performing RT-PCR on said isolated RNA.
12 more elements
Dependencies are inferred from the claim text.