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US 8,771,975 B2Granted
Quantitation of GCP activity in biological samples
AssigneeThe Johns Hopkins University
InventorsBarbara S. Slusher, Michael Polydefkis, Camilo Rojas, Marigo Stathis
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Abstract
Provided herein are methods for quantitating glutamate carboxypeptidase II activity in a biological sample, such as a skin biopsy sample, and for determining whether an agent is inhibiting GCP in a subject.
Claims20
A method of quantitating glutamate carboxypeptidase II (GCPII) activity in a biological skin sample that contains endogenous N-acetyl-aspartate glutamate (NAAG), the method comprising the steps of:
- (a) reducing the amount of the endogenous NAAG in a biological skin sample;
- (b) contacting the biological skin sample with exogenous NAAG;
- and
- (c) detecting an exogenous NAAG cleavage product in the biological skin sample.
1 more element
A method of determining whether an agent is inhibiting GCPII in a subject, the method comprising the steps of:
- (a) administering the agent to the subject;
- (b) obtaining a biological skin sample from the subject;
- (c) reducing the amount of endogenous NAAG in the biological skin sample;
- (d) contacting the biological skin sample with exogenous NAAG;
- and
- (e) detecting the amount of an exogenous NAAG cleavage product in the biological skin sample;
- wherein a lower amount of the exogenous NAAG cleavage product in the biological skin sample compared to the amount of exogenous NAAG cleavage product in a control skin sample indicates that the agent is inhibiting GCPII in the subject.
4 more elements
Dependencies are inferred from the claim text.