Clozapine immunoassay
AssigneeSaladax Biomedical Inc.
InventorsSalvatore J. Salamone, Jodi Blake Courtney, Howard Sard, Christopher Spedaliere
Life of this patent
Estimating the term…
Abstract
Novel conjugates and immunogens derived from clozapine and antibodies generated by these immunogens are useful in immunoassays for the quantification and monitoring of clozapine in biological fluids.
Claims15
An immunoassay for detecting clozapine in a sample comprising providing a mixture of a) said sample, b) an antibody selectively reactive with clozapine and not substantially cross-reactive with N-desmethylclozapine and clozapine-N-oxide, and c) a conjugate of a carrier having either a reactive thiol or amino group with a compound of the formula:
- wherein B is:
- —(C═O)—, —(C═O)—NH—, —(C═O)—O—, —CH2-;
- Y is an organic spacing group;
- X is a terminal functional group capable of binding to said carrier;
- and
- p is an integer from 0 to 1;
- or salts thereof;
- causing the clozapine in the sample and said conjugate in said mixture to bind with said antibody and thereafter measuring the amount of said conjugate in said mixture which is bound or unbound to said antibody whereby the presence of clozapine in the sample can be determined.
A kit for determining the presence of clozapine in a patient sample comprising reagents in separate containers, one of the reagents being a conjugate of a carrier having either a reactive thiol or amino group with a compound of the formula:
- wherein B is:
- —(C═O)—, —(C═O)—NH—, —(C═O)—O—, —CH2—;
- Y is an organic spacing group;
- X is a terminal functional group capable of binding to said carrier;
- and
- p is an integer from 0 to 1;
- or salts thereof;
- and the second container containing an antibody selectively reactive with clozapine and not substantially cross-reactive with N-desmethylclozapine and clozapine-N-oxide.
Dependencies are inferred from the claim text.