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US 8,771,955 B2Granted
Device and method for extraction and analysis of nucleic acids from biological samples
AssigneeBlood Cell Storage, Inc.
InventorsMichael W. Reed, Oliver Z. Nanassy
Life of this patent
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Abstract
Device and methods for extracting and analyzing nucleic acids from biological samples.
Claims12
A method for quantifying and amplifying the amount of nucleic acids in a sample, comprising:
- (a) contacting nucleic acids from a sample with a nucleic acid amplification reaction mixture comprising a first fluorescent compound having a first fluorescence emission maximum, a second fluorescent compound having a second fluorescence emission maximum, and a third fluorescent compound having a third fluorescence emission maximum and a fluorescence intensity dependent on the concentration of amplified nucleic acids, wherein the first, second, and third fluorescence emission maxima are different, each having a fluorescence intensity dependent on the concentration of nucleic acids;
- (b) measuring the fluorescence of the first fluorescent compound to determine the amount of nucleic acids;
- (c) treating the nucleic acids and the nucleic acid amplification reaction mixture under conditions for amplifying the nucleic acids to provide amplified nucleic acids;
- and
- (d) measuring the fluorescence of the first fluorescent compound to determine the amount of amplified nucleic acids.
2 more elements
A method for quantifying and amplifying the amount of nucleic acids in a sample, comprising:
- (a) contacting nucleic acids from a sample with a first fluorescent compound having a first fluorescence emission maximum and a fluorescence intensity dependent on the concentration of nucleic acids;
- (b) measuring the fluorescence of the first fluorescent compound to determine the amount of nucleic acids;
- (c) treating the nucleic acids with a nucleic acid amplification reaction mixture under conditions for amplifying the nucleic acids to provide amplified nucleic acids, wherein the nucleic acid amplification reaction mixture comprises a second fluorescent compound having a second fluorescence emission maximum and a fluorescence intensity dependent on the concentration of nucleic acids, and a third fluorescent compound having a third fluorescence emission maximum and a fluorescence intensity dependent on the concentration of nucleic acids, wherein the first, second, and third fluorescence emission maxima are different;
- and
- (d) measuring the fluorescence of the second fluorescent compound to determine the presence of a gene or gene product.
2 more elements
Dependencies are inferred from the claim text.