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US 8,771,939 B2Granted
Method for methylation analysis of nucleic acid
AssigneeEpigenomics AG
InventorsReimo Tetzner, Joern Lewin
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Abstract
The present invention relates to a method for methylation analysis. It comprises the providing of a double stranded nucleic acid; its conversion, whereby unmethylated bases become distinguishable in their base-pairing behavior from methylated bases, and the analysis of both of the converted nucleic acid strands.
Claims7
A method for methylation analysis of a nucleic acid, comprising
- providing a double stranded nucleic acid,
- converting said nucleic acid in such a way that 5-methylcytosine remains unchanged, while unmethylated cytosine is converted to uracil or to another base that is distinguished by cytosine in its base-pairing behavior, said reaction leading to two different converted nucleic acid strands that are no longer complementary to each other,
- analyzing both of the converted nucleic acid strands, wherein one of the two strands is analyzed in a methylation specific manner and the other of the two strands is analyzed in a non-methylation specific manner, wherein
- (i) the methylation-specific manner comprises the analysis of the presence or absence of methylation of one or more cytosines;
- and
- (ii) the non-methylation specific manner comprises the analysis of the copy number, deletion or amplification of one or more adjacent nucleotides in a corresponding, overlapping, adjacent or different section of the second strand or an analysis of SNP.
3 more elements
Dependencies are inferred from the claim text.