Fusion proteins and their use in the diagnosis and treatment of leishmaniasis
AssigneeInfectious Disease Research Institute
InventorsAjay Bhatia, Steven G. Reed
Life of this patent
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Abstract
The present invention relates generally to a fusion protein made from a synthetic gene construct comprising of elements derived from the Leishmania antigens K26, K39, and K9. The fusion protein is particularly useful in the diagnosis of leishmaniasis, particularly visceral leishmaniasis in animals such as humans and dogs.
Claims22
A diagnostic kit for detecting asymptomatic or sub-clinical visceral leishmaniasis in a biological sample selected from the group consisting of sera, blood, and saliva, comprising:
- (a) an fusion polypeptide comprising Leishmania antigens K26, K39, and K9, wherein the Leishmania antigen K26 comprises a sequence having at least 98% identity to SEQ ID NO:5, the Leishmania antigen K39 comprises a sequence having at least 98% identity to SEQ ID NO:6, and the Leishmania antigen K9 comprises a sequence having at least 98% identity to SEQ ID NO:7;
- and
- (b) a detection reagent.
A diagnostic kit for identifying a patient afflicted with asymptomatic or sub-clinical visceral leishmaniasis that is likely to develop acute visceral leishmaniasis, comprising:
- (a) an fusion polypeptide comprising Leishmania antigens K26, K39, and K9, wherein the Leishmania antigen K26 comprises a sequence having at least 98% identity to SEQ ID NO:5, the Leishmania antigen K39 comprises a sequence having at least 98% identity to SEQ ID NO:6, and the Leishmania antigen K9 comprises a sequence having at least 98% identity to SEQ ID NO:7;
- (b) a polypeptide comprising an amino acid sequence as set forth in SEQ ID NOs:10 or 11;
- and
- (c) a detection reagent.
An isolated fusion polypeptide comprising (i) a Leishmania antigen K26 comprising a sequence having at least 98% identity to SEQ ID NO:5;
- (ii) a Leishmania antigen K39 comprising a sequence having at least 98% identity to SEQ ID NO:6;
- and (iii) a Leishmania antigen K9 comprising a sequence having at least 98% identity to SEQ ID NO:7.
An isolated fusion polypeptide comprising an amino acid sequence having at least 95% identity to amino acid residues 10-262 of SEQ ID NO:8.
Dependencies are inferred from the claim text.