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US 8,771,708 B2Granted
Biologically-active radiolabeled Cry1Fa and receptor binding assay methods
AssigneeDow AgroSciences LLC
InventorsSteven L. Evans, Jianquan Li, Joel J. Sheets
Life of this patent
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Abstract
Cysteine-specific radiolabeled Cry1Fa protein retains insecticidal activity against insect pests and binds to insect brush border membrane vesicle receptors in a saturable manner. The biologically-active radiolabeled Cry1Fa protein is useful in competitive binding assays with other Cry toxins.
Claims2
A Cry1Fa holotoxin labeled with radioiodinated fluorescein-5-maleimide at position C205, said radiolabeled holotoxin retaining the ability to selectively bind to receptors in brush border membrane vesicles prepared from midguts of Spodoptera exigua and Spodoptera frugiperda.
Dependencies are inferred from the claim text.