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US 8,771,693 B2Granted
Methods and compositions for the generation and use of conformation-specific antibodies
AssigneeBeth Israel Deaconess Medical Center, Inc.
InventorsKun Ping Lu, Xiao Zhen Zhou
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Abstract
The present invention features methods and compositions for the generation and use of conformation-specific anti-bodies or fragments thereof.
Claims20
An isolated conformation-specific antibody or fragment thereof that specifically binds to a Xaa-Pro motif of a polypeptide, wherein:
- said polypeptide is a PPlase substrate, the peptidyl-prolyl bond of said Xaa-Pro motif is in a cis conformation, said Xaa is serine or threonine and is phosphorylated, and said antibody or fragment thereof binds to the cis conformation of said Xaa-Pro motif of said polypeptide with at least 10- to 100-fold greater affinity than to the trans conformation of said Xaa-Pro motif of said polypeptide.
An isolated conformation-specific antibody or fragment thereof that specifically binds to a Xaa-Pro motif of a polypeptide, wherein:
- said polypeptide is a PPlase substrate, the peptidyl-prolyl bond of said Xaa-Pro motif is in a trans conformation, said Xaa is serine or threonine and is phosphorylated, and said antibody or fragment thereof binds to the trans conformation of said Xaa-Pro motif of said polypeptide with at least 10- to 100-fold greater affinity than to the cis conformation of said Xaa-Pro motif of said polypeptide.
Dependencies are inferred from the claim text.