Methods of using adipose tissue-derived cells in augmenting autologous fat transfer

A method of augmenting autologous fat transfer to a subject, comprising:

• removing a first portion of adipose tissue that comprises a cell population comprising adipose-derived stem cells and progenitor from said subject;
• introducing the removed adipose tissue into a self-contained cell processing unit configured to maintain a closed pathway;
• isolating said cell population that comprises adipose-derived stem cells and progenitor cells from mature adipocytes and connective tissue present in the removed adipose tissue so as to obtain a substantially isolated cell population comprising disaggregated adipose-derived stem cells and progenitor cells, while maintaining a closed pathway;
• concentrating said substantially isolated cell population comprising disaggregated adipose-derived stem cells so as to obtain a concentrated cell population that comprises disaggregated adipose-derived stem cells and progenitor cells, while maintaining a closed pathway;
• mixing said concentrated cell population comprising adipose-derived stem cells and progenitor cells with a second portion of unprocessed adipose tissue comprising intact, non-disaggregated tissue fragments obtained from said subject, so as to obtain an adipose tissue composition comprising a mixture of said concentrated cell population comprising disaggregated adipose-derived stem cells and progenitor cells and said unprocessed adipose tissue comprising intact, non-disaggregated tissue fragments tissue;
• and
• reintroducing said adipose tissue composition into said subject.